Example 2 - Increase of Biotechnology Production Effectivenes

Industrial production of drugs and/or their components is based very often on the work of an enzyme which catalyses the reaction of their synthesis. Drug production in the industrial quantities with the assistance of enzymes requires often the optimization of process in the reactor. Indeed on the several steps of production cycle several by-products, which pollute final product and increase its yield might be produced concurrently with the main product. Furthermore enzyme which catalyzes the reaction of product formation may be inactivated or works nonoptimally in the conditions of industrial production. Thus, the competent control of process which takes place in the reactor on the every step of technological cycle is required for increase of production effectiveness. It may be fulfilled by using of special program product which should answer at least two main requirements  - take into consideration all the features of industrial process and account in detail the mechanism of enzyme action. The method developed takes all these factors into account.

PACS_0_eng

Problem: to optimize conditions of enzymatic production of 6-aminopenicillan acid in bioreactor

Subject of inquiry: industrial biotechnological processes of 6-aminopenicillan acid production.

Approach:

  • To develop and verify kinetic model of enzyme penicillin acylase.
  • To predict optimal conditions of enzymatic biotechnological synthesis of 6-aminopenicillan acid.
  • Based on the penicillin acylase kinetic model and its predictions to develop advisory software allowing to control of biotechnological process.
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Result №1. Kinetic model of penicillin acylase have been developed and verified against in vitro experimental data.
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Result №2. Based on the analysis of the model behavior we have predicted optimal conditions of enzyme catalyzed synthesis of 6-aminopenicillan acid.
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Result №3. Based on the penicillin acylase kinetic model and its predictions we have developed advisory software (PACS) allowing to control of biotechnological process.

Conclusions

On the basis of kinetic model of penicillin acylase developed and technological features of biotechnological synthesis of 6-aminopenicillan acid we have developed software (PACS). In this software all requirements of biotechnologists who deal with control of 6-aminopenicillan acid production in industrial conditions have been completely realized. Software algorithm allows both to regulate reaction conditions and to give to the biotechnologist the optimal strategy of components adding. That allows to reach the maximal efficiency of the biotechnological process. Implementation of the software has led to the enzyme savings amounted about 10% and to the considerable increase of the relative yield of product. It is important that obtained results have not only completely repaid research efforts but also led to the significant profit.


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